gut proteolytic profile of larval callosobruchus maculatus (coleoptera: chrysomelidae) in response to feeding on different fabaceous host plants

نویسندگان

s. sarboland

department of plant protection, faculty of agriculture, urmia university, urmia, islamic republic of iran. f. mehrkhou

department of plant protection, faculty of agriculture, urmia university, urmia, islamic republic of iran. m. imani

department of basic sciences (biochemistry), faculty of veterinary, urmia university, urmia, islamic republic of iran.

چکیده

the impact of three different fabaceous host plants including cowpea (vigna unguiculata), chickpea (cicer arietinum), and mung bean (vigna radiata) seeds was investigated using biochemical approaches on possible changes of gut proteolytic activity of the cowpea weevil, callosobruchus maculatus at 30±1˚c and 70±5% rh and a photoperiod of 8:16 (l:d). results revealed that ph of 4-5 and 9 was optimal for the activity of larval gut proteases using azocasein and hemoglobin as general substrates. different serine (bapna, saapfpna, pmsf, tlck, and tpck) and cysteine (z-arg-arg- pna, z-phe-arg-pna and dtt) specific substrates inhibitors and activator were used as a further proof of the proteolytic profile in the gut of c. maculates. although combinations of serine and cysteine proteases were observed, the cysteine proteases had the highest rate on the studied hosts. the protease activity, especially cystein protease, was the highest on cowpea, which was supported by hemoglobin (0.156±0.045 u mg-1), z-phe-arg-pna (2.85 u mg-1) substrates and dtt (90.00±0.10%) as an activator. due to the importance and frequency of cysteine proteinases and their effects on biological and physiological process, it would be better to design pest management programs based on cysteine plant proteinase inhibitors as transgenic plants.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Inbreeding depression in two seed-feeding beetles, Callosobruchus maculatus and Stator limbatus (Coleoptera: Chrysomelidae).

Inbreeding depression is well documented in insects but the degree to which inbreeding depression varies among populations within species, and among traits within populations, is poorly studied in insects other than Drosophila. Inbreeding depression was examined in two long-term laboratory colonies of the seed beetle, Callosobruchus maculatus (Fabricius), which are used frequently as models for...

متن کامل

Egg-Dumping Behavior Is Not Correlated With Wider Host Acceptance in the Seed Beetle Callosobruchus maculatus (Coleoptera: Chrysomelidae: Bruchinae)

If typical host plants are absent, some herbivorous insects “dump” eggs on unsuitable substrates, even though this can cause complete larval mortality and reduced maternal life span. In the seedbeetleCallosobruchusmaculatus (F.) (Coleoptera:Chrysomelidae: Bruchinae), the tendency to dump eggs varies genetically both within and between populations. A previous study hypothesized that egg-dumping ...

متن کامل

Potential of four tropical plant powders as grain protectants against Callosobruchus maculatus (Coleoptera: Chrysomelidae)

Effects of four tropical plant (Aframomum melegueta, Zanthoxylum zanthoxyloides, Piper guineense and Eugenia aromatica) were investigated under tropical laboratory storage conditions for the protection of cowpea seeds against insect infestation. The plant materials were pulverised into fine powder after air drying and admixed with 20 g of cowpea seeds at the ...

متن کامل

nutritional indices of the willow leaf beetle, plagiodera versicolora (coleoptera: chrysomelidae) on different host plants

nutritional indices of the willow leaf beetle, plagiodera versicolora laicharting, third instar larvae and adults were studied on four host plants including salix alba l., salix aegyptica l., populus caspica bornm.and populus alba l.at 22 ± 2 ºc, 70 ± 10% r.h., and a photoperiod of 16:8 h (l: d). the results showed that the highest consumed food by the larvae and adults (148.5 ± 18.0 and 175.21...

متن کامل

Bioactivity of Novel Botanical Insecticide From Gnidia kaussiana (Thymeleaceae) Against Callosobruchus maculatus (Coleoptera: Chrysomelidae) in Stored Vigna subterranea (Fabaceae) Grains

Hexane, acetone, and methanol extracts from Gnidia kaussiana Meisn (Thymeleaceae), each at two dosages (0.2 and 1 ml/50 g grains corresponding, respectively to 1 and 5g/kg), and neem seed oil (NSO), used as standard insecticide were evaluated for repellence, toxicity to Callosobruchus maculatus (F.) (Coleoptera: Chrysomelidae) adults, F1 progeny inhibition, persistence and as grain protectant d...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
journal of agricultural science and technology

جلد ۱۹، شماره ۱، صفحات ۱۲۱-۱۳۲

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023