gut proteolytic profile of larval callosobruchus maculatus (coleoptera: chrysomelidae) in response to feeding on different fabaceous host plants
نویسندگان
چکیده
the impact of three different fabaceous host plants including cowpea (vigna unguiculata), chickpea (cicer arietinum), and mung bean (vigna radiata) seeds was investigated using biochemical approaches on possible changes of gut proteolytic activity of the cowpea weevil, callosobruchus maculatus at 30±1˚c and 70±5% rh and a photoperiod of 8:16 (l:d). results revealed that ph of 4-5 and 9 was optimal for the activity of larval gut proteases using azocasein and hemoglobin as general substrates. different serine (bapna, saapfpna, pmsf, tlck, and tpck) and cysteine (z-arg-arg- pna, z-phe-arg-pna and dtt) specific substrates inhibitors and activator were used as a further proof of the proteolytic profile in the gut of c. maculates. although combinations of serine and cysteine proteases were observed, the cysteine proteases had the highest rate on the studied hosts. the protease activity, especially cystein protease, was the highest on cowpea, which was supported by hemoglobin (0.156±0.045 u mg-1), z-phe-arg-pna (2.85 u mg-1) substrates and dtt (90.00±0.10%) as an activator. due to the importance and frequency of cysteine proteinases and their effects on biological and physiological process, it would be better to design pest management programs based on cysteine plant proteinase inhibitors as transgenic plants.
منابع مشابه
Evaluation of feeding response,age specific survival and longevity of poplar leafbeetle chrysomela populi (coleoptera chrysomelidae) on five host plants
متن کامل
Inbreeding depression in two seed-feeding beetles, Callosobruchus maculatus and Stator limbatus (Coleoptera: Chrysomelidae).
Inbreeding depression is well documented in insects but the degree to which inbreeding depression varies among populations within species, and among traits within populations, is poorly studied in insects other than Drosophila. Inbreeding depression was examined in two long-term laboratory colonies of the seed beetle, Callosobruchus maculatus (Fabricius), which are used frequently as models for...
متن کاملEgg-Dumping Behavior Is Not Correlated With Wider Host Acceptance in the Seed Beetle Callosobruchus maculatus (Coleoptera: Chrysomelidae: Bruchinae)
If typical host plants are absent, some herbivorous insects “dump” eggs on unsuitable substrates, even though this can cause complete larval mortality and reduced maternal life span. In the seedbeetleCallosobruchusmaculatus (F.) (Coleoptera:Chrysomelidae: Bruchinae), the tendency to dump eggs varies genetically both within and between populations. A previous study hypothesized that egg-dumping ...
متن کاملPotential of four tropical plant powders as grain protectants against Callosobruchus maculatus (Coleoptera: Chrysomelidae)
Effects of four tropical plant (Aframomum melegueta, Zanthoxylum zanthoxyloides, Piper guineense and Eugenia aromatica) were investigated under tropical laboratory storage conditions for the protection of cowpea seeds against insect infestation. The plant materials were pulverised into fine powder after air drying and admixed with 20 g of cowpea seeds at the ...
متن کاملnutritional indices of the willow leaf beetle, plagiodera versicolora (coleoptera: chrysomelidae) on different host plants
nutritional indices of the willow leaf beetle, plagiodera versicolora laicharting, third instar larvae and adults were studied on four host plants including salix alba l., salix aegyptica l., populus caspica bornm.and populus alba l.at 22 ± 2 ºc, 70 ± 10% r.h., and a photoperiod of 16:8 h (l: d). the results showed that the highest consumed food by the larvae and adults (148.5 ± 18.0 and 175.21...
متن کاملBioactivity of Novel Botanical Insecticide From Gnidia kaussiana (Thymeleaceae) Against Callosobruchus maculatus (Coleoptera: Chrysomelidae) in Stored Vigna subterranea (Fabaceae) Grains
Hexane, acetone, and methanol extracts from Gnidia kaussiana Meisn (Thymeleaceae), each at two dosages (0.2 and 1 ml/50 g grains corresponding, respectively to 1 and 5g/kg), and neem seed oil (NSO), used as standard insecticide were evaluated for repellence, toxicity to Callosobruchus maculatus (F.) (Coleoptera: Chrysomelidae) adults, F1 progeny inhibition, persistence and as grain protectant d...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
journal of agricultural science and technologyجلد ۱۹، شماره ۱، صفحات ۱۲۱-۱۳۲
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023